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High-throughput Genotyping
NGS-based target enrichment genotyping service for animal and plant research
Overview
For Molecular Breeding, the Availability and Easy Accessibility of Genomic Resources is a Prerequisite
Celemics introduces sequencing service solution for plant and animal research with NGS-based high-throughput genotyping.
We have utilized NGS and hybridization-based capture technology, whereby a high number of regions of interest are simultaneously enriched using specifically designed probe, to provide new insights into different agricultural genomics research.
Features & Benefits
NGS-based Target Enrichment
Sequencing Assay
Utilizing NGS-based target enrichment method for higher accuracy and cost-effective experiment compared to conventional methods such as conventional GBS, PCR, and microarray
Comprehensive Analysis with High Accuracy
Perform comprehensive assay of 100 to 10,000 markers with minimized false-negative and false-positives as well as for discovering novel SNPs
Outstanding Performance Regardless of Various Origins
Receive high-quality results enabled by species-specifically designed blocking oligos across all types of origins
Maximized Analytic Efficiency
Benefit from Celemics’ library preparation kits, target capture technology, and multiplexing indices specifically designed for high-throughput genotyping
Comparison with Conventional Technologies
| Advantages | Disadvantage | |
|---|---|---|
| Conventional GBS | 1. Sequencing of multiple samples due to lower amount of data required compared to WGS | 1. Limited biomarkers available due to limited conserved regions, reducing overall resolution 2. Unable to detect SNPs in the restriction sites |
| Microarray | 1. Higher reproducibility than conventional>GBS | 1. Hard to customize new targets (novel biomarkers) 2. Low flexibility to meet various kinds of genotyping |
| PCR | 1. Cost-effective for low number of samples 2. Easy and fast analysis | 1. Limited number of biomarkers to analyze at once 2. Inappropriate for mass-analysis of biomarkers |
| Celemics Target Enrichment | 1. Cost saving : Highly cost-effective when assessing multiple samples
2. Flexible customization : Novel biomarkers can be added or removed 3. Comprehensive analysis : Including novel SNP discovery 4. Exceptional performance : Celemics proprietary blocking oligo design technology 5. Wide compatibility : Compatible with a wide range of sample types |
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