This video serves as a supplementary tool for the protocol employing CeleMag™ Clean-up Beads for size selection. Following the Adapter ligation steps using the Celemics Library Preparation Kit for the IonTorrent sequencing platform, it is recommended to proceed with DNA Library size selection. The necessary materials for these steps include CeleMag™ Clean-up Beads, freshly made 80% ethanol, nuclease-free water, and consumables.
Key moments:
0:14 Introduction | Sample Size Selection
0:53 Consumables and Equipment
01:03 Step 1: Sample Purification using CeleMag™ Clean-up Beads
04:52 Step 2: Sample Size Selection using CeleMag™ Clean-up Beads
Proceed directly to the next step of Amplification of the Adapter-Ligated Library. If immediate continuation is not possible, store the sample at -20°C. This video is provided by Celemics technical support. Thank you for watching.
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[Video Manual] Size Selection of Sample Using CeleMag™ Clean up Bead
In-silico designed and wet-lab validated to ensure state-of-the-art capture performance