This video serves as a supplementary tool for customers using Celemics NGS Library Prep kit for IonTorrent Sequencing Platform. You will need Library Preparation box 1 and 2, as well as CeleMag Clean-up Bead and CLM Polymerase.
🧬CELEMICS’ Library Preparation Kit🧬
All Celemics library preparation kits are highly optimized for efficient usage with Celemics ready-to-use or customized panels. Both the Standard and Enzymatic Preparation (EP) kits include end-repair and A-tailing enzyme mix index primers (single or dual), adapters and buffer.
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Key moments:
0:37 Step 1. DNA Fragmentation
01:33 Step 2. (Optional) Purification of sample using CeleMag Clean-up Bead
05:33 Step 3. End Repair of DNA
07:23 Step 4. Adapter Ligation
10:35 Step 5-1. Purification of sample using CeleMag Clean-up Bead
14:36 Step 5-2. DNA Library Size Selection
14:48 Step 6. Amplification of the Adapter-Ligated Library
16:54 Step 7. Purification of sample using CeleMag Clean-up Bead
21:02 Assess Quality and Quantity
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