In this video, we delve into the complexities of Next Generation Sequencing (NGS) to address a common challenge known as Bubble Product.
If you’re using platforms like Illumina or MGI, this is a must-watch for you!
✅ How Bubbles Form in DNA Libraries: Learn the factors that may lead to bubble formation, such as the amount of input DNA, number of PCR cycles, and DNA quality.
✅ Why Bubbles are a Problem: Understand how bubbles impact the accuracy and integrity of DNA library concentration and size measurements, and how they can lead to biased results in Target Enrichment experiments.
✅ How to Prevent Bubbles: Find out key strategies to prevent bubble formation, including optimal DNA input amounts, DNA fragmentation times, and adjusting PCR cycles.
Celemics provide the best solutions for Library Preparation and Target Enrichment kits to help you achieve optimal experimental conditions and accurate results. Stay connected with us for more tips and insights!
✅ Webpage: https://www.celemics.com/
✅ Contact Us: https://www.celemics.com/company/cont…
Key moments:
0:10 Introduction
0:24 Bubble Products in NGS?
0:50 How Bubbles Form in DNA Libraries
1:50 Why Bubbles are a Problem
2:31 How to Prevent Bubbles
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