Blogs

PDF Download Next Generation Sequencing (NGS), particularly short-read NGS using platforms like Illumina and MGI, requires adapter sequences attached to both ends of the DNA library. Consequently, all samples have identical sequences at both ends. These identical sequences can cause a byproduct known as Bubble product during library preparation, leading to various experimental and sequencing […]

Next-Generation Sequencing (NGS) has become an essential tool in genomics research and also plays a crucial role in variant analysis. Setting the appropriate sequencing depth is critical when analyzing variants using NGS data, as it determines the accuracy and cost-effectiveness of the experiment. For instance, higher sequencing depth allows for the detection of variants with […]

The United States Food and Drug Administration (FDA) has recently announced a final rule over Laboratory Developed Tests (LDTs), which are a critical component of in vitro diagnostic systems. This regulatory shift emphasizes the importance of precise and reliable diagnostic methods in healthcare. LDTs refer to tests that are conceived, designed, and executed within a […]

Maximizing the efficiency of NGS library preparation is particularly crucial when working with limited sample quantities. Biological samples from patients, especially those like FFPE or cfDNA, may have limited amounts of DNA, making it challenging to use sufficient amount of DNA for NGS analysis. Therefore, using high-efficiency DNA library preparation kit for subsequent experiments such […]

Evaluating the performance of a target enrichment panel can be achieved using numerous metrics, one of which is uniformity, which assesses how evenly data are obtained across target regions. In ideal targeted sequencing, an equal amount of data would be obtained from all capture regions. However, in reality, discrepancies in sequencing depth occur across regions […]

  Next-generation sequencing (NGS) procedures require library preparation steps, PCR, purification, and quantification. To enhance procedural efficiency, Celemics introduces the Celemics Normalization Bead (CeleNM™ Bead), featuring a streamlined protocol with reduced hands-on time and superior performance.   Celemics Normalization Bead (CeleNM™ Bead) Library preparation kits from various vendors typically involve PCR followed by purification and […]