Robustness of Celemics polymerase for GC-rich or AT-rich Samples
Introduction | Expanding NGS Beyond Human Genomes
Recently, more and more customers have inquired whether Celemics’ kits can be used for various organisms beyond humans, such as viruses and bacteria. These organisms often have genomic DNA with high or very low GC-ratios, which may affect experimental outcomes, and some customers have expressed concerns about this.
In this post, we present experimental data to demonstrate how reliably and evenly the Celemics polymerase amplifies libraries even from GC-rich and AT-rich genomic DNA samples.
Experimental Setup | Conditions and Organisms
- Genomic DNA used:
1) Bacillus cereus (AT-rich)
2) Caulobacter segnis (GC-rich) - Input DNA: 50 ng
- Library Preparation Kit: Celemics Enzymatic Library Preparation Kit
- Sequencing Platform: Illumina NextSeq 500 system
1. Results | Amplification of AT-rich Genome (Bacillus Cereus, GC contents: 35.3%)
Thegreen histogram in the graph below shows the distribution of GC content calculated by dividing the Bacillus cereus genome into 50 bp blocks and counting how many blocks fall into each GC ratio range. The results confirm that Bacillus cereus has an AT-rich profile. The “Read Ratio” on the left indicates how evenly each block was amplified compared to the average, with values closer to 1 representing ideal amplification.
Using libraries prepared with Celemics’ Enzymatic Library Preparation Kit, amplification was performed using three polymerases, including that of Celemics. The results showed that the Celemics polymerase exhibited comparable performance to leading competitors in uniformly amplifying AT-rich regions.
2. Results | Amplification of GC-rich Genome (Caulobacter Segnis, GC contents: 67.7%)
The same experiment was conducted under identical conditions using the GC-rich Caulobacter segnis genome. The results showed that the Celemics polymerase provided more uniform amplification even in high-GC regions compared to competitor products.
Conclusion | Reliable Performance Across Diverse Genomes
These experimental results confirm that the Celemics polymerase can amplify DNA with excellent uniformity across both GC-rich and AT-rich regions, outperforming competitors. If you require library preparation for analyzing the genomes of various organisms, we encourage you to experience Celemics’ NGS solutions.hese results demonstrate that Celemics’s Double-Stranded cDNA Synthesis Kit can produce consistent, high-quality ds-cDNA, largely unaffected by variables such as the experimenter, sample amount, or challenging sample types. This enables researchers to perform reliable analysis using a broader range of samples.
CLM Polymerase for Reliable NGS Library Preparation
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