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Enzymatic Long read Library Preparation Kit for ONT Platforms

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[NEW PRODUCT LAUNCH: Celemics Enzymatic Long-read Library Preparation Kit]
Celemics Enzymatic NGS Library Preparation Kit for Oxford Nanopore Technologies

The demand for long-read sequencing continues to grow, enabling researchers to overcome limitations of short-read technologies, especially in resolving structural variations, repetitive regions, and complex genomes. To support this expanding field, Celemics introduces a new all-in-one solution tailored for Oxford Nanopore Technologies (ONT):

Celemics Enzymatic Long-read Library Preparation Kit

Designed for Long-read Sequencing

Celemics Enzymatic Long-read Library Preparation Kit is designed to generate long-insert libraries with an average size of ~2 kb, optimized for Oxford Nanopore sequencing. It enables researchers to prepare high-quality libraries and perform target enrichment without the need for DNA fragmentation or size selection instruments.
Key benefits include:

  • Enzymatic Fragmentation & Size Optimization
  • No Need for Mechanical Shearing and Size Selection Instruments
  • Simple Workflow — Hands-on Time under 1.5 hours
  • High Target Specificity for Confident Variant Detection

Streamlined Workflow

The experimental protocol closely follows Celemics’ widely adopted short-read library preparation workflow, making it easier for users to transition to long-read sequencing without additional training. Workflow diagram of the Celemics Enzymatic Long-read Library Preparation Kit and Target Enrichment process, showing steps from enzymatic preparation to post-PCR.

Workflow diagram of the Celemics Enzymatic Long-read Library Preparation and Target Enrichment Kit, showing steps from enzymatic preparation to post-PCR.

 

Workflow Overview:

  1. Enzymatic Fragmentation, End Repair, & A-tailing
  2. Adapter Ligation
  3. Pre-PCR & QC
  4. Hybridization-based Target Enrichment
  5. Post-PCR & QC

Researchers benefit from a cost-effective and intuitive protocol, requiring no specialized equipment or consumables for DNA fragmentation or size selection.

Proven Performance

Post-hybridization sequencing results have shown high target specificity, reinforcing the robustness of the enrichment process even in a long-read context. This makes the kit suitable for applications requiring confident variant detection, such as:

  • Pharmacogenomics
  • Structural variant analysis
  • Repetitive region sequencing
  • Custom gene panels

Flexible Options with Custom Panels

Celemics provides customized target enrichment panels to suit a wide range of research applications.

With the launch of this ONT-optimized Long-read NGS kit, Celemics strengthens its commitment to providing platform-flexible, user-friendly, and cost-effective NGS solutions. Whether you are already using short-read platforms or expanding into long-read technologies, this kit offers a smooth transition and robust performance.

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