Celemics Library Preparation Kit & Polymerase: Effect of High/Low GC-Ratio
To conduct Next-Generation Sequencing (NGS), DNA sample must undergo a Library Preparation process tailored to the specific sequencing platform. Additionally, to perform further experiments like Target Enrichment, PCR is required to amplify the quantity of the library. However, the efficiency of this PCR process can be affected by the ratio of G/C bases in the sample DNA.
CELEMICS offers a Library Prep Kit and Polymerase that minimize the effect of sample GC content on results, ensuring that valuable samples are not wasted. In this post, we’ll examine the effects of varying GC ratios on library preparation using CELEMICS’ Library Prep Kit and Polymerase, with two case studies featuring different GC content levels.
Comparing Sequencing Depth Distribution by GC Ratio
To validate our product performance, we conducted a comparative experiment using two strains of gDNA with low and high GC content, respectively, and the results are presented as below.
* Following CELEMICS’ standard library prep protocol, we sequenced on the Illumina NextSeq 500 platform. To assess GC-related effects, the genome was divided into regions of equal size, calculating each region’s GC ratio and standardizing sequencing depth across these regions.
Our findings demonstrate that when using CELEMICS’ Library Prep Kit and Polymerase, a GC content between 15% and 85% provide consistently even library yields.
For further information on how our reliable products can benefit your research, please contact us at support@celemics.com.
