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Maximizing the efficiency of NGS library preparation is particularly crucial when working with limited sample quantities. Biological samples from patients, especially those

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Maximizing the efficiency of NGS library preparation is particularly crucial when working with limited sample quantities. Biological samples from patients, especially those like FFPE or cfDNA, may have limited amounts of DNA, making it challenging to use sufficient amount of DNA for NGS analysis. Therefore, using high-efficiency DNA library preparation kit for subsequent experiments such […]

Evaluating the performance of a target enrichment panel can be achieved using numerous metrics, one of which is uniformity, which assesses how evenly data are obtained across target regions. In ideal targeted sequencing, an equal amount of data would be obtained from all capture regions. However, in reality, discrepancies in sequencing depth occur across regions […]

  Next-generation sequencing (NGS) procedures require library preparation steps, PCR, purification, and quantification. To enhance procedural efficiency, Celemics introduces the Celemics Normalization Bead (CeleNM™ Bead), featuring a streamlined protocol with reduced hands-on time and superior performance.   Celemics Normalization Bead (CeleNM™ Bead) Library preparation kits from various vendors typically involve PCR followed by purification and […]

  Next-generation sequencing (NGS) technology is widely used across various fields for generating large-scale genomic data at a cost-effective rate. Due to the vast amount of data produced in a single sequencing run, index sequences are employed to distinguish and allocate data for each sample. This allows for simultaneous sequencing of multiple samples in single […]

The Workflow of NGS Library Preparation Kit Celemics provides the library preparation kit for customers using Illumina, MGI, and Thermo Fisher Ion Torrent NGS platforms. There are two types of Celemics’ exclusive library preparation kits for all three platforms: LP kit and EP kit. This post aims to introduce Celemics’ products and workflow of our […]

The genome, which contains the genetic information of an organism, comprises various combinations of A, T, G, C nucleotides. Depending on these sequences’ characteristics, experiments and analyses might face challenges, impacting the accuracy of sequencing and analysis. Therefore, it is crucial to consider the characteristics and aspects of these so-called ‘hard-to-capture’ regions when designing a […]