Methylation Sequencing Panel
Facilitate Epigenetics research with Celemics’ NGS solution
Overview
Methylation Sequencing
Methylation sequencing can identify the locations and levels of methylated DNA in a genome-wide or targeted manner for various applications of epigenetic research. Celemics’ solution for DNA methylation sequencing can provide more comprehensive and detailed patterns of either genome-wide or specific target region of your choice.
Standard Workflow of Targeted Methyl-seq
Standard Workflow of Targeted Methyl-seq
Standard Workflow of Targeted Methyl-seq
DNA
Sample
Methylated
adaptor
On-bead
conversion
protocol
Index PCR
Post-PCR
Library
Preparation
Bisulfite
Conversion
Target
Enrichment
Sequencing
Targeted
Methyl-seq Data
Celemics Proprietary Probe Design for Methyl-seq
Celemics Proprietary Probe Design for Methyl-seq
“G/A” panel
“C/T” panel
Pre-PCR
Bisulfite
conversion
Celemics Proprietary Probe Design for Methyl-seq
Features & Benefits
Probe Specifically Designed for Methyl-seq
Celemics introduces elaborate design considering the sequence alteration by bisulfite conversion. We perform comparison analysis of the sequences before and after bisulfite conversion, enabling accurate detection of methylation sites.
BI analysis for methylation sequencing
BI analysis for methylation sequencing
Alignment
FASTQ Filter
Methylation
Extractor
Visualization
Methylation
Pattern
Deduplication
Raw
Data
Bismark
Standard Analysis Pipeline Targeted Methyl-seq
Compatible with Various Sample Types and C/T Conversion Method
Along with proprietary probes and exclusive workflow for methyl-seq, this panel allows methylation analysis from various sample sources, including gDNA and cfDNA.
As method for bisulfite conversion preference can be diverse, we also offer a market leading quality of panel that is compatible with both enzyme or chemical C/T conversion method; no compromised sequencing data depending on the conversion method
High Reproducibilty of Methylation Pattern Analysis
The results demonstrate high reproducibility of the analysis, yielding the same methylation patterns when repeatedly tested with the identical specimens.
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Resources
Technical Resources
Safety Data Sheets
References
Epigenomics
Identification of tissue of origin in cancer of unknown primary using a targeted bisulfite sequencing panel
Bae JM, Ahn JY, Lee H, Jang H, Han H, Jeong J, et al. Identification of tissue of origin in cancer of unknown primary using a targeted bisulfite sequencing panel. Epigenomics. 2022 May;14(10):615–28.
10.2217/epi-2021-0477
Investig Clin Urol
Germline pathogenic variants in unselected Korean men with prostate cancer
So MK, Ahn HK, Huh J, Kim KH. Germline pathogenic variants in unselected Korean men with prostate cancer. Investig Clin Urol. 2022 May;63(3):294–300.
DOI 10.4111/icu.20220044
Scientific Reports
Feasibility of targeted cascade genetic testing in the family members of BRCA1/2 gene pathogenic variant/likely pathogenic variant carriers
Lee J, Ham JY, Park HY, Jung JH, Kim WW, Kang B, et al. Feasibility of targeted cascade genetic testing in the family members of BRCA1/2 gene pathogenic variant/likely pathogenic variant carriers. Sci Rep. 2022 Feb 3;12(1):1842.
DOI 10.1038/s41598-022-05931-3
BMC Medical Genomics
A novel bi-alleleic DDX41 mutations in B-cell lymphoblastic leukemia: case report
Shin WY, Yoon SY, Park R, Kim JA, Song HH, Bang HI, et al. A novel bi-alleleic DDX41 mutations in B-cell lymphoblastic leukemia: case report. BMC Med Genomics. 2022 Mar 4;15(1):46.
DOI 10.1186/s12920-022-01191-2