Methylation Sequencing Panel
Facilitate Epigenetics research with Celemics’ NGS solution
Overview
Methylation Sequencing
Methylation sequencing can identify the locations and levels of methylated DNA in a genome-wide or targeted manner for various applications of epigenetic research. Celemics’ solution for DNA methylation sequencing can provide more comprehensive and detailed patterns of either genome-wide or specific target region of your choice.
Standard Workflow of Targeted Methyl-seq
Standard Workflow of Targeted Methyl-seq
Standard Workflow of Targeted Methyl-seq
DNA
Sample
Methylated
adaptor
On-bead
conversion
protocol
Index PCR
Post-PCR
Library
Preparation
Bisulfite
Conversion
Target
Enrichment
Sequencing
Targeted
Methyl-seq Data
Celemics Proprietary Probe Design for Methyl-seq
Celemics Proprietary Probe Design for Methyl-seq
“G/A” panel
“C/T” panel
Pre-PCR
Bisulfite
conversion
Celemics Proprietary Probe Design for Methyl-seq
Probe Specifically Designed for Methyl-seq
Celemics introduces elaborate design considering the sequence alteration by bisulfite conversion. We perform comparison analysis of the sequences before and after bisulfite conversion, enabling accurate detection of methylation sites.
BI analysis for methylation sequencing
BI analysis for methylation sequencing
Alignment
FASTQ Filter
Methylation
Extractor
Visualization
Methylation
Pattern
Deduplication
Raw
Data
Bismark
Standard Analysis Pipeline Targeted Methyl-seq
Compatible with Various Sample Types and C/T Conversion Method
Along with proprietary probes and exclusive workflow for methyl-seq, this panel allows methylation analysis from various sample sources.
As method for bisulfite conversion preference can be diverse, we also offer a market leading quality of panel that is compatible with both enzyme or chemical C/T conversion method; no compromised sequencing data depending on the conversion method
High Reproducibilty of Methylation Pattern Analysis
The results demonstrate high reproducibility of the analysis, yielding the same methylation patterns when repeatedly tested with the identical specimens.
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Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Customization to Next Level; tailored NGS panel customization and assay optimization
Resources
Technical Resources
Safety Data Sheets
References
Cancer Research and Treatment
Varlitinib and Paclitaxel for EGFR/HER2 Co-Expressing Advanced Gastric Cancer: A Multicenter Phase Ib/II Study (K-MASTER-13)
Koo DH, Jung M, Kim YH, Jeung HC, Zang DY, Bae WK, Kim H, Kim HS, Lee CK, Kwon WS, Chung HC. Varlitinib and Paclitaxel for EGFR/HER2 Co-Expressing Advanced Gastric Cancer: a Multicenter Phase Ib/II Study (K-MASTER-13). Cancer Research and Treatment. 2024 Apr 29.
10.4143/crt.2023.1324
Cancers
Discovery and Validation of Survival-Specific Genes in Papillary Renal Cell Carcinoma Using a Customized Next-Generation Sequencing Gene Panel
Hwang J, Bang S, Choi MH, Hong SH, Kim SW, Lee HE, Yang JH, Park US, Choi YJ. Discovery and Validation of Survival-Specific Genes in Papillary Renal Cell Carcinoma Using a Customized Next-Generation Sequencing Gene Panel. Cancers. 2024 Jan;16(11):2006.
10.3390/cancers16112006
Frontiers in Neurology
Case report: Compound heterozygous variants detected by next-generation sequencing in a Tunisian child with ataxia-telangiectasia
Ammous-Boukhris N, Abdelmaksoud-Dammak R, Ben Ayed-Guerfali D, Guidara S, Jallouli O, Kamoun H, Charfi Triki C, Mokdad-Gargouri R. Case report: Compound heterozygous variants detected by next-generation sequencing in a Tunisian child with ataxia-telangiectasia. Frontiers in Neurology. 2024 May 31;15:1344018.
10.3389/fneur.2024.1344018
Scientific Reports
Sex-specific survival gene mutations are discovered as clinical predictors of clear cell renal cell carcinoma
Hwang J, Lee HE, Han JS, Choi MH, Hong SH, Kim SW, Yang JH, Park U, Jung ES, Choi YJ. Sex-specific survival gene mutations are discovered as clinical predictors of clear cell renal cell carcinoma. Scientific Reports. 2024 Jul 9;14(1):15800.
10.1038/s41598-024-66525-9