Metagenomic Sequencing Panel
Detection of Bacterial Pathogen and Microbe Classification
Overview
Metagenomic Sequencing
Celemics’ Metagenomic Sequencing panel allows for the characterization and differentiation of a myriad of microbial species.
The 16S V4 (or V3-V4) region of bacteria and archaea and 18S ITS1 (or ITS1-ITS2) region of fungi are amplified by PCR, and has indices and adapters attached after clean-up using CeleMag Beads for NGS and bioinformatics analysis. Various analysis reports are provided through Celemics’ robust analysis pipeline according to the customer’s study purposes.
Features & Benefits
Flexible Panel Design
At Celemics, we can offer both ready-to-use and flexible custom panel design options that best suits the purpose for individual customer. For the most popular metagenomic sequencing regions, we have panel with V3 or V3-V4 region for sequencing bacteria available, along with various customizable options and for fungal sequencing, we can offer ready-to-use panel set of ITS1 or ITS1-ITS2 region.
16S rRNA sequencing for bacteria
18S ITS sequencing for fungi
Support Bioinformatics Analysis for Classification and Diversity
By Celemics’ bioinformatics analysis pipeline, various types of analysis for the microbes are supported including taxonomy classification and diversity. As a company with our exclusive BI pipeline, we offer analysis result in diverse formats for comprehensive metagenomic insights.
Example of metagenomic sequencing analysis report
Results presented above are a few selected examples of the metagenomics sequencing results that Celemics provides. Contact us for more information.
Taxonomy analysis
Alpha rarefaction
Alpha diversity
Beta diversity
Workflow
NGS-based metagenomic analysis workflow
- By Celemics’ BI analysis pipeline, various types of analysis for the microbes are supported including taxonomy classification and diversity.
Raw Sequences
Representative
sequences
Taxonomy
classification
Diversity analyses
Feature table
Denoising
Raw Sequences
Denoising
Representative
sequences
Feature table
Taxonomy
classification
Diversity
analyses
Related Products
Library Preparation Kit
Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
CAS
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
CeleMag Clean-up Bead
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
CLM Polymerase
Market-leading yield and accuracy with minimized PCR bias. Includes all reaction components for PCR
Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Market-leading yield and accuracy with minimized PCR bias. Includes all reaction components for PCR
Resources
Technical Resources
Safety Data Sheets
References
Foods
Characterisation of Lactobacillus plantarum of Dairy-Product Origin for Probiotic Chèvre Cheese Production
Ngamsomchat A, Kaewkod T, Konkit M, Tragoolpua Y, Bovonsombut S, Chitov T. Characterisation of Lactobacillus plantarum of Dairy-Product Origin for Probiotic Chèvre Cheese Production. Foods. 2022 Mar 24;11(7):934.
DOI 10.3390/foods11070934
Bioscience of Microbiota, Food and Health
Investigation of gut microbiota and short-chain fatty acids in Strongyloides stercoralis-infected patients in a rural community
Nguyen HT, Hongsrichan N, Intuyod K, Pinlaor P, Yingklang M, Chaidee A, et al. Investigation of gut microbiota and short-chain fatty acids in Strongyloides stercoralis-infected patients in a rural community. Biosci Microbiota Food Health. 2022;41(3):121–9.
DOI 10.12938/bmfh.2021-054
Pedosphere
Metagenetics of fairy rings reveals complex and variable fungal communities
Xu S, Dong K, Lee S, Ogwu MC, Undrakhbold S, Singh D, et al. Metagenetics of fairy rings reveals complex and variable fungal communities. Pedosphere [Internet]. 2022 Jun 7 [cited 2023 Feb 20]; Available from: https://www.sciencedirect.com/science/article/pii/S1002016022000492
DOI 10.1016/j.pedsph.2022.06.043
J Fungi
First Isolation of Pseudogymnoascus destructans, the Fungal Causative Agent of White-Nose Syndrome, in Korean Bats (Myotis petax)
Kim YS, Yang MS, Jeong CG, Chung CU, Oem JK. First Isolation of Pseudogymnoascus destructans, the Fungal Causative Agent of White-Nose Syndrome, in Korean Bats (Myotis petax). J Fungi (Basel). 2022 Oct 12;8(10):1072.
DOI 10.3390/jof8101072