High-throughput Genotyping
NGS-based target enrichment methods for higher genotyping efficiency in plant and animal research
Overview
For Molecular Breeding, the Availability and Easy Accessibility of Genomic Resources is a Prerequisite
Although technological advances have provided a range of resources like molecular markers, genetic linkage maps, whole genome sequences, and transcriptomes, agricultural genomics has been facing many challenges.
Celemics provides the solution with our high-throughput genotyping panel.
We have utilized NGS and hybridization-based capture technology, whereby a high number of regions of interest are simultaneously enriched using specifically designed probe, to provide new insights into different agricultural genomics research.
Features & Benefits
NGS-based Target Enrichment
Sequencing Assay
Utilizing NGS-based target enrichment method for higher accuracy and cost-effective experiment compared to conventional methods such as conventional GBS, PCR, and microarray
Comprehensive Analysis with High Accuracy
Perform comprehensive assay of 100 to 10,000 markers with minimized false-negative and false-positives as well as for discovering novel SNPs
Outstanding Performance Regardless of Various Origins
Receive high-quality results enabled by species-specifically designed blocking oligos across all types of origins
Maximized Analytic Efficiency
Benefit from Celemics’ library preparation kits, target capture technology, and multiplexing indices specifically designed for high-throughput genotyping
Comparison with Conventional Technologies
| Advantages | Disadvantage | |
|---|---|---|
| Conventional GBS | 1. Sequencing of multiple samples due to lower amount of data required compared to WGS | 1. Limited biomarkers available due to limited conserved regions, reducing overall resolution 2. Unable to detect SNPs in the restriction sites |
| Microarray |
1. Higher reproducibility than conventional
GBS |
1. Hard to customize new targets (novel biomarkers) 2. Low flexibility to meet various kinds of genotyping |
| PCR | 1. Cost-effective for low number of samples 2. Easy and fast analysis | 1. Limited number of biomarkers to analyze at once 2. Inappropriate for mass-analysis of biomarkers |
| Celemics Target Enrichment | 1. Cost saving : Highly cost-effective when assessing multiple samples
2. Flexible customization : Novel biomarkers can be added or removed 3. Comprehensive analysis : Including novel SNP discovery 4. Exceptional performance : Celemics proprietary blocking oligo design technology 5. Wide compatibility : Compatible with a wide range of sample types |
Related Products
Customized NGS Panel
Customization to Next Level; tailored NGS panel customization and assay optimization
Library Preparation Kit
Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
CAS
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
CeleMag Clean-up Bead
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Customization to Next Level; tailored NGS panel customization and assay optimization
Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
References
Epigenomics
Identification of tissue of origin in cancer of unknown primary using a targeted bisulfite sequencing panel
Bae JM, Ahn JY, Lee H, Jang H, Han H, Jeong J, et al. Identification of tissue of origin in cancer of unknown primary using a targeted bisulfite sequencing panel. Epigenomics. 2022 May;14(10):615–28.
10.2217/epi-2021-0477
Investig Clin Urol
Germline pathogenic variants in unselected Korean men with prostate cancer
So MK, Ahn HK, Huh J, Kim KH. Germline pathogenic variants in unselected Korean men with prostate cancer. Investig Clin Urol. 2022 May;63(3):294–300.
DOI 10.4111/icu.20220044
Scientific Reports
Feasibility of targeted cascade genetic testing in the family members of BRCA1/2 gene pathogenic variant/likely pathogenic variant carriers
Lee J, Ham JY, Park HY, Jung JH, Kim WW, Kang B, et al. Feasibility of targeted cascade genetic testing in the family members of BRCA1/2 gene pathogenic variant/likely pathogenic variant carriers. Sci Rep. 2022 Feb 3;12(1):1842.
DOI 10.1038/s41598-022-05931-3
BMC Medical Genomics
A novel bi-alleleic DDX41 mutations in B-cell lymphoblastic leukemia: case report
Shin WY, Yoon SY, Park R, Kim JA, Song HH, Bang HI, et al. A novel bi-alleleic DDX41 mutations in B-cell lymphoblastic leukemia: case report. BMC Med Genomics. 2022 Mar 4;15(1):46.
DOI 10.1186/s12920-022-01191-2