High-throughput Genotyping
NGS-based target enrichment methods for higher genotyping efficiency in plant and animal research
Overview
For Molecular Breeding, the Availability and Easy Accessibility of Genomic Resources is a Prerequisite
Although technological advances have provided a range of resources like molecular markers, genetic linkage maps, whole genome sequences, and transcriptomes, agricultural genomics has been facing many challenges.
Celemics provides the solution with our high-throughput genotyping panel.
We have utilized NGS and hybridization-based capture technology, whereby a high number of regions of interest are simultaneously enriched using specifically designed probe, to provide new insights into different agricultural genomics research.
NGS-based Target Enrichment
Sequencing Assay
Utilizing NGS-based target enrichment method for higher accuracy and cost-effective experiment compared to conventional methods such as conventional GBS, PCR, and microarray
Comprehensive Analysis with High Accuracy
Perform comprehensive assay of 100 to 10,000 markers with minimized false-negative and false-positives as well as for discovering novel SNPs
Outstanding Performance Regardless of Various Origins
Receive high-quality results enabled by species-specifically designed blocking oligos across all types of origins
Maximized Analytic Efficiency
Benefit from Celemics’ library preparation kits, target capture technology, and multiplexing indices specifically designed for high-throughput genotyping
Comparison with Conventional Technologies
| Advantages | Disadvantage | |
|---|---|---|
| Conventional GBS | 1. Sequencing of multiple samples due to lower amount of data required compared to WGS | 1. Limited biomarkers available due to limited conserved regions, reducing overall resolution 2. Unable to detect SNPs in the restriction sites |
| Microarray | 1. Higher reproducibility than conventional GBS | 1. Hard to customize new targets (novel biomarkers) 2. Low flexibility to meet various kinds of genotyping |
| PCR | 1. Cost-effective for low number of samples 2. Easy and fast analysis | 1. Limited number of biomarkers to analyze at once 2. Inappropriate for mass-analysis of biomarkers |
| Celemics Target Enrichment | 1. Cost saving : Highly cost-effective when assessing multiple samples
2. Flexible customization : Novel biomarkers can be added or removed 3. Comprehensive analysis : Including novel SNP discovery 4. Exceptional performance : Celemics proprietary blocking oligo design technology 5. Wide compatibility : Compatible with a wide range of sample types |
Related Products
Customization to Next Level; tailored NGS panel customization and assay optimization
Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Customization to Next Level; tailored NGS panel customization and assay optimization
Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
References
Molecular Cancer
Distant origin of glioblastoma recurrence: neural stem cells in the subventricular zone serve as a source of tumor reconstruction after primary resection
Li, X., Kim, H. J., Yoo, J., Lee, Y., Nam, C. H., Park, J., … & Lee, J. H. (2025). Distant origin of glioblastoma recurrence: neural stem cells in the subventricular zone serve as a source of tumor reconstruction after primary resection. Molecular Cancer, 24(1), 64.
10.1186/s12943-025-02273-2
Frontiers in Oncology
The clinical relevance of surgical specimens for RNA sequencing in lung cancer: a cohort study
Eom, J. S., Kim, S. H., Kim, K., Kim, A., Ahn, H. Y., Mok, J., … & Kim, M. H. (2024). The clinical relevance of surgical specimens for RNA sequencing in lung cancer: A cohort study. Frontiers in Oncology, 14, 1462519.
10.3389/fonc.2024.1462519
Cancer Research and Treatment
Varlitinib and Paclitaxel for EGFR/HER2 Co-Expressing Advanced Gastric Cancer: A Multicenter Phase Ib/II Study (K-MASTER-13)
Koo DH, Jung M, Kim YH, Jeung HC, Zang DY, Bae WK, Kim H, Kim HS, Lee CK, Kwon WS, Chung HC. Varlitinib and Paclitaxel for EGFR/HER2 Co-Expressing Advanced Gastric Cancer: a Multicenter Phase Ib/II Study (K-MASTER-13). Cancer Research and Treatment. 2024 Apr 29.
10.4143/crt.2023.1324
Cancers
Discovery and Validation of Survival-Specific Genes in Papillary Renal Cell Carcinoma Using a Customized Next-Generation Sequencing Gene Panel
Hwang J, Bang S, Choi MH, Hong SH, Kim SW, Lee HE, Yang JH, Park US, Choi YJ. Discovery and Validation of Survival-Specific Genes in Papillary Renal Cell Carcinoma Using a Customized Next-Generation Sequencing Gene Panel. Cancers. 2024 Jan;16(11):2006.
10.3390/cancers16112006