African Swine Fever Virus
Specifically designed to Identify various strains of ASFV in a single NGS reaction
Overview
NGS for African Swine Fever Virus
Despite the fact that high morbidity and mortality of African swine fever (ASF) has a severe impact on the global swine industry, there are no current effective treatments or vaccines commercially available.
In order to effectively identify and diagnose the African Swine Fever virus, Celemics introduces ASFV panel which can identify 26 strains of genotype II virus in a single NGS run.
With Celemics’ ASFV panel, we can rapidly distinguish the cause and route of infection, helping in early prevention of wide spread of the disease.
Comprehensive Analysis of ASFV Subtypes
Celemics’ ASFV Panel can detect genotype II virus subtypes with our exclusive in-house designed hybridization probes. It can provide highly accurate results even from swine blood sample, which is considered more challenging due to its lower viral load compared to concentrated culture supernatant or spleen tissue sample. We have optimized the panel and reagents for your convenient and effective testing.
The panel validation result shows high uniformity and high coverage at all levels
Mean depth coverage uniformity
Coverage
Swine-specific Blocking Reagent
As NGS experts, we understand the importance of blocking oligo and how it can affect the sequencing results. In order to provide the most effective sequencing results, Celemics has incorporated our proprietary technology to design and provide swine-specific blocking reagent that efficiently filters out repetitive sequences, allowing for the selective retrieval of ASFV sequences
With the same sequencing amount, target enrichment NGS yielded 29% virus reads out of a total of 1,039,018 reads, while whole genome sequencing (WGS) yielded 0.5% virus reads (green) out of a total of 39,261 reads.
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Sequencing
Analysis
with BI tools
Capture
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Capture
Sequencing
Analysis
with BI tools
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Capture
Sequencing
Analysis
with BI tools
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Capture
Sequencing
Analysis
with BI tools
Specification
| Target viruses* | ASFV 26 strains |
|---|---|
| Target size | 192 kb |
| Mutation type | Virus detection, Virus genome assembly |
| Sample type (amount) | Swine blood (> 50 ng of fragmented DNA) |
| Platform | All sequencers from Illumina, Thermo Fisher, MGI, PacBio, and Oxford Nanopore |
| Bioinformatics Support | Celemics Virus Verifier (Detection report) |
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Stabilize RNA to double-stranded cDNA for unquestionable performance
Customization to Next Level; tailored NGS panel customization and assay optimization
End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Resources
Technical Resources
Safety Data Sheets
If you require the latest MSDS file, please contact us via ‘Contact Us‘.
MSDS_African Swine Fever Virus Panel_Illumina
MSDS_African Swine Fever Virus Panel_Thermo Fisher
MSDS_African Swine Fever Virus Panel_MGI
References
Scientific Reports
Natural fatal infection of Tembusu virus in bottlenose dolphins in Thailand
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10.1038/s41598-025-93477-5
PLOS ONE
Molecular epidemiology and phylogenetic insights of lumpy skin disease in cattle from diverse agro-ecological regions of Punjab, Pakistan
Jabbar, M. H., Atif, F. A., Kashif, M., Ahmed, I., Iarussi, F., & Swelum, A. A. (2025). Molecular epidemiology and phylogenetic insights of lumpy skin disease in cattle from diverse agro-ecological regions of Punjab, Pakistan. PloS one, 20(1), e0315532.
10.1371/journal.pone.0315532
Journal of Global Antimicrobial Resistance
Therapeutic potential of novel phages with antibiotic combinations against ESBL-producing and carbapenem-resistant Escherichia Coli
Shamsuzzaman, M., Kim, S., & Kim, J. (2025). Therapeutic Potential of Novel Phages with Antibiotic Combinations Against ESBL-Producing and Carbapenem-Resistant Escherichia coli. Journal of Global Antimicrobial Resistance.
10.1016/j.jgar.2025.04.005
International Journal of Arrhythmia
Clinical role of genetic testing for the Brugada syndrome overlapping with arrhythmogenic cardiomyopathy
Jeong, J. H., Lee, H. S., Choi, Y. Y., Kim, Y. G., Shim, J., Hwang, J. H., … & Choi, J. I. (2024). Clinical role of genetic testing for the Brugada syndrome overlapping with arrhythmogenic cardiomyopathy. International Journal of Arrhythmia, 25(1), 12.
10.1186/s42444-024-00121-w