African Swine Fever Virus
Specifically designed to Identify various strains of ASFV in a single NGS reaction
Overview
NGS for African Swine Fever Virus
Despite the fact that high morbidity and mortality of African swine fever (ASF) has a severe impact on the global swine industry, there are no current effective treatments or vaccines commercially available.
In order to effectively identify and diagnose the African Swine Fever virus, Celemics introduces ASFV panel which can identify 26 strains of genotype II virus in a single NGS run.
With Celemics’ ASFV panel, we can rapidly distinguish the cause and route of infection, helping in early prevention of wide spread of the disease.
Features & Benefits
Comprehensive Analysis of ASFV Subtypes
Celemics’ ASFV Panel can detect genotype II virus subtypes with our exclusive in-house designed hybridization probes. It can provide highly accurate results even from swine blood sample, which is considered more challenging due to its lower viral load compared to concentrated culture supernatant or spleen tissue sample. We have optimized the panel and reagents for your convenient and effective testing.
The panel validation result shows high uniformity and high coverage at all levels
Mean depth coverage uniformity
Coverage
Swine-specific Blocking Reagent
As NGS experts, we understand the importance of blocking oligo and how it can affect the sequencing results. In order to provide the most effective sequencing results, Celemics has incorporated our proprietary technology to design and provide swine-specific blocking reagent that efficiently filters out repetitive sequences, allowing for the selective retrieval of ASFV sequences
With the same sequencing amount, target enrichment NGS yielded 29% virus reads out of a total of 1,039,018 reads, while whole genome sequencing (WGS) yielded 0.5% virus reads (green) out of a total of 39,261 reads.
Workflow
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Sequencing
Analysis
with BI tools
Capture
Workflow
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Capture
Sequencing
Analysis
with BI tools
Workflow
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Capture
Sequencing
Analysis
with BI tools
Workflow
- Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.
DNA Library
Preparation
Hybridization
Capture
Sequencing
Analysis
with BI tools
Specification
| Target viruses* | ASFV 26 strains |
|---|---|
| Target size | 192 kb |
| Mutation type | Virus detection, Virus genome assembly |
| Sample type (amount) | Swine blood (> 50 ng of fragmented DNA) |
| Platform | All sequencers from Illumina, Thermo Fisher, MGI, PacBio, and Oxford Nanopore |
| Bioinformatics Support | Celemics Virus Verifier (Detection report) |
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Resources
Technical Resources
Safety Data Sheets
References
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SARS-CoV-2 in hospital air as revealed by comprehensive respiratory viral panel sequencing
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DOI 10.1016/j.infpip.2021.100199
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SARS-CoV-2 Transmission from Human to Pet and Suspected Transmission from Pet to Human, Thailand
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DOI 10.1128/jcm.01058-22
Food Environ Virol
Detection of Norovirus Recombinant GII. 2 [P16] Strains in Oysters in Thailand
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DOI 10.1007/s12560-022-09508-1
Sci Rep
Whole-genome analysis and mutation pattern of SARS-CoV-2 during first and second wave outbreak in Gwangju, Republic of Korea
Chatterjee S, Kim CM, Lee YM, Seo JW, Kim DY, Yun NR, et al. Whole-genome analysis and mutation pattern of SARS-CoV-2 during first and second wave outbreak in Gwangju, Republic of Korea. Sci Rep. 2022 Jul 5;12(1):11354.
DOI 10.1038/s41598-022-14989-y