African Swine Fever Virus

Specifically designed to Identify various strains of ASFV in a single NGS reaction

Overview

NGS for African Swine Fever Virus

Despite the fact that high morbidity and mortality of African swine fever (ASF) has a severe impact on the global swine industry, there are no current effective treatments or vaccines commercially available.

In order to effectively identify and diagnose the African Swine Fever virus, Celemics introduces ASFV panel which can identify 26 strains of genotype II virus in a single NGS run.

With Celemics’ ASFV panel, we can rapidly distinguish the cause and route of infection, helping in early prevention of wide spread of the disease.

African Swine Fever Virus
Features & Benefits

Comprehensive Analysis of ASFV Subtypes

Celemics’ ASFV Panel can detect genotype II virus subtypes with our exclusive in-house designed hybridization probes. It can provide highly accurate results even from swine blood sample, which is considered more challenging due to its lower viral load compared to concentrated culture supernatant or spleen tissue sample. We have optimized the panel and reagents for your convenient and effective testing.

The panel validation result shows high uniformity and high coverage at all levels

Comprehensive Analysis of ASFV Subtypes

Mean depth coverage uniformity

Coverage

Swine-specific Blocking Reagent

As NGS experts, we understand the importance of blocking oligo and how it can affect the sequencing results. In order to provide the most effective sequencing results, Celemics has incorporated our proprietary technology to design and provide swine-specific blocking reagent that efficiently filters out repetitive sequences, allowing for the selective retrieval of ASFV sequences

With the same sequencing amount, target enrichment NGS yielded 29% virus reads out of a total of 1,039,018 reads, while whole genome sequencing (WGS) yielded 0.5% virus reads (green) out of a total of 39,261 reads.

Swine-specific Blocking Reagent
Workflow
  • Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.

DNA Library
Preparation

Hybridization

Sequencing

Analysis
with BI tools

Capture

african swine fever virus - workflow
Workflow
  • Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.

DNA Library

Preparation

Hybridization

Capture

Sequencing

Analysis
with BI tools

african swine fever virus - workflow
Workflow
  • Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.

DNA Library

Preparation

Hybridization

Capture

Sequencing

Analysis
with BI tools

african swine fever virus - workflow
Workflow
  • Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information, leading to a cost-effective experimental experience by preventing additional NGS operation.

DNA Library

Preparation

Hybridization

Capture

Sequencing

Analysis
with BI tools

african swine fever virus - workflow

Specification

*Gene Add-On Service: Genes can be added by customer’s request.
Target viruses* ASFV 26 strains
Target size 192 kb
Mutation type Virus detection, Virus genome assembly
Sample type (amount) Swine blood (> 50 ng of fragmented DNA)
Platform All sequencers from Illumina, Thermo Fisher, MGI, PacBio, and Oxford Nanopore
Bioinformatics Support Celemics Virus Verifier (Detection report)

Related Products

related-product

Stabilize RNA to double-stranded cDNA for unquestionable performance

Customized NGS Panel

Customization to Next Level; tailored NGS panel customization and assay optimization

Celemics Analysis Service

End-to-end complete Bioinformatics solution; Trust CAS with your BI analysis

Streptavidin Bead

Incomparable quality magnetic bead for simple, flexible, and reproducible purification

Resources

Technical Resources

[Product Sheet] Celemics Agrigenomics Panels

[Product Overview] Celemics Innovative NGS Solutions for Agrigenomics

[Product Overview] African Swine Fever Virus Panel

[Best Practice] African Swine Fever Virus Panel

Celemics Target Enrichment Panel Overview

Celemics Products & Services

Safety Data Sheets

MSDS_African Swine Fever Virus Panel_Illumina

MSDS_African Swine Fever Virus Panel_Thermo Fisher

MSDS_African Swine Fever Virus Panel_MGI

References

Infect Prev Pract

SARS-CoV-2 in hospital air as revealed by comprehensive respiratory viral panel sequencing

Habibi N, Uddin S, Behbehani M, Abdul Razzack N, Zakir F, Shajan A. SARS-CoV-2 in hospital air as revealed by comprehensive respiratory viral panel sequencing. Infect Prev Pract. 2022 Mar;4(1):100199.

 

DOI 10.1016/j.infpip.2021.100199


View Detail >

J Clin Microbiol

SARS-CoV-2 Transmission from Human to Pet and Suspected Transmission from Pet to Human, Thailand

Piewbang C, Poonsin P, Lohavicharn P, Wardhani SW, Dankaona W, Puenpa J, et al. SARS-CoV-2 Transmission from Human to Pet and Suspected Transmission from Pet to Human, Thailand. J Clin Microbiol. 2022 Nov 16;60(11):e0105822.

 

DOI 10.1128/jcm.01058-22


View Detail >

Food Environ Virol

Detection of Norovirus Recombinant GII. 2 [P16] Strains in Oysters in Thailand

Kittigul L, Pombubpa K, Rupprom K, Thasiri J. Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand. Food Environ Virol. 2022 Mar;14(1):59–68.

 

DOI 10.1007/s12560-022-09508-1


View Detail >

Sci Rep

Whole-genome analysis and mutation pattern of SARS-CoV-2 during first and second wave outbreak in Gwangju, Republic of Korea

Chatterjee S, Kim CM, Lee YM, Seo JW, Kim DY, Yun NR, et al. Whole-genome analysis and mutation pattern of SARS-CoV-2 during first and second wave outbreak in Gwangju, Republic of Korea. Sci Rep. 2022 Jul 5;12(1):11354.

 

DOI 10.1038/s41598-022-14989-y


View Detail >