Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Library Preparation Kit
Optimized for high-efficiency Celemics Panels (Standard / EP)
Overview
NGS Library Preparation Kit
Celemics Library Preparation Kits are optimized for high-efficiency NGS library construction across all Celemics panels. The Standard Library Prep Kit (LP Kit, Mechanical Fragmentation) and the Enzymatic Prep Kit (EP Kit) provide a complete reagent set – including an End-Repair/A-tailing enzyme mix, index primers (single or dual), adapters, and buffers – to streamline your workflow. These kits enable researchers, hospitals, and diagnostics companies to achieve maximal library yields, exceptional reproducibility, and robust sequencing performance in a convenient, single-vendor solution.
Library Preparation Workflow for
NGS Target Enrichment
The Standard Kit follows a conventional workflow using mechanical (ultrasonication) or enzymatic fragmentation (fragmentase), followed by end-repair, A-tailing, adapter ligation, and indexing PCR. In contrast, the EP Kit integrates DNA fragmentation into the End-Repair/A-tailing step, eliminating the need for a separate fragmentation step. This flexibility allows users to leverage existing shearing instruments or adopt the simplified EP protocol, depending on their lab setup.
Library preparation workflow
For Option 1, ultra sonicator is not provided with the kit.
For Option 2, the inclusion of the fragmentase in the kit is optional.
NGS Library Preparation | ||
|---|---|---|
Standard Fragmentation | EP Fragmentation | |
| Option 1. Sonication | Option 2. Fragmentase | FER / A |
| Bead Purification & Quantification | ||
| ER / A | ER / A | |
| Adapter Ligation (Single / Dual Index) | ||
| Bead Purification | ||
| Index PCR | ||
Target Enrichment | ||
High Yield and
Exceptional Reproducibility
Celemics Library Prep Kits are designed for maximum DNA library yield and consistency.
In comparative evaluations, both the Standard and EP Kits delivered the highest amplified library yields among major vendor kits.
Library Yield Reproducibility of LP and EP Kits
Five replicate libraries were prepared using 100 ng of input DNA to evaluate the reproducibility and yield performance of the Celemics LP and EP Kits. The LP Kit was tested with 8 PCR cycles and the EP Kit with 5 PCR cycles under identical conditions.
Both kits demonstrated consistent library yields across replicates, confirming high reproducibility, while the EP Kit showed higher overall yield efficiency.More uniform read depths over GC-rich regions compared to competitor products.
Uniform Amplification across
high and low-GC regions
Celemics Library Preparation Kits deliver reliable amplification performance across a wide range of GC contents. Powered by Celemics’ proprietary CLM Polymerase, the kits maintain exceptional amplification uniformity and minimize PCR bias in both GC-rich and GC-poor genomic regions.
In comparative studies using bacterial genomes with varying GC compositions, the CLM Polymerase demonstrated steady and balanced read coverage, ensuring high-quality sequencing data regardless of sequence composition.
Amplification of high-GC and low-GC regions using CLM Polymerase (EP Kit)
The amplification capacity was tested using bacterial samples with known GC contents. The results show that CLM Polymerase allows steady and uniform amplification without PCR bias for both high-GC and low-GC regions.
* For GC-ratio analysis, the genomic DNA sequence was partitioned into contiguous 200 bp fragments. The GC content of each fragment was computed, and the resulting values were aggregated into a histogram with 2% interval bins. The green, red, and purple lines represent the normalized sequencing depth across different GC-ratio regions.
Broad Compatibility
with Sequencing Platforms
Celemics LP and EP Kits are platform-agnostic and validated on all major NGS instruments.
The kits are tailored for seamless integration with Illumina , MGI sequencing platforms, and Thermo Fisher’s Ion Torrent sequencers. This broad compatibility allows you to adopt Celemics’ library prep solutions without altering your existing sequencing setup or analysis pipeline. When combined with Celemics’ Target Enrichment Panels, users can leverage a fully optimized end-to-end workflow from sample to sequencer.
All-in-One Convenience and
Automation-Friendly
Celemics provides all necessary components in a single kit, eliminating the need to source additional reagents and simplifying protocol setup. Indexing primers are available in single or dual index formats to support different throughput and multiplexing needs. The workflow is fully automation-compatible and can be implemented on common liquid-handling robots (such as Hamilton, Tecan, or Beckman systems) for high-throughput, walk-away processing. This all-in-one, automation-friendly design saves labor and time while ensuring consistency, making it ideal for both small labs and large clinical or core facilities
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Resources
Technical Resources
Safety Data Sheets
If you require the latest MSDS file, please contact us via ‘Contact Us‘.
MSDS_Library Preparation Kit_Illumina
MSDS_Library Preparation Kit_Thermo Fisher
MSDS_Library Preparation Kit_MGI