Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Home » PRODUCTS » Modular Accessories »
Library Preparation Kit
Optimized for high-efficiency Celemics Panels (Standard / EP)
Overview
Library Preparation Kit
All Celemics library preparation kits are highly optimized for efficient usage with Celemics ready-to-use or customized panels. Both the Standard and Enzymatic Preparation (EP) kits include end-repair and A-tailing enzyme mix index primers (single or dual), adapters and buffer.
Workflow
Library Preparation Workflow for NGS Target Enrichment
Celemics provides two methods for library preparation step, Standard Library Preparation Kit and Enzymatic Preparation Kit (EP Kit). For Standard Library Preparation Kit (Standard Kit),, customers can use ultra-sonication devices or fragmentase for DNA fragmentation. The Enzymatic Preparation Kit (EP Kit) EP Kit includes all steps from enzymatic fragmentation to ER/A in a single reaction enabling convenient workflow. EP kit does not need of purification step, allowing for minimal DNA loss.
Library preparation workflow
For Option 1, ultra sonicator is not provided with the kit.
For Option 2, the inclusion of the fragmentase in the kit is optional.
NGS Library Preparation |
||
|---|---|---|
Standard Fragmentation |
EP Fragmentation |
|
| Option 1. Sonication | Option 2. Fragmentase | FER / A |
| Bead Purification & Quantification | ||
| ER / A | ER / A | |
| Adapter Ligation (Single / Dual Index) | ||
| Bead Purification | ||
| Index PCR | ||
Target Enrichment |
||
Related Products
CeleMag Clean-up Bead
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
Streptavidin Beads
High biotin-streptavidin binding capacity with superior performance to selectively isolate target genes bound to hybridization probes during target enrichment process
CLM Polymerase
Market-leading yield and accuracy with minimized PCR bias. Includes all reaction components for PCR
Clinical Exome Sequencing
Comprehensive detection of clinically significant variants for diverse genetic diseases
Incomparable quality magnetic bead for simple, flexible, and reproducible purification
High biotin-streptavidin binding capacity with superior performance to selectively isolate target genes bound to hybridization probes during target enrichment process
Market-leading yield and accuracy with minimized PCR bias. Includes all reaction components for PCR
Comprehensive detection of clinically significant variants for diverse genetic diseases
Resources
Technical Resources
Safety Data Sheets
If you require the latest MSDS file, please contact us via ‘Contact Us‘.
MSDS_Library Preparation Kit_Illumina
MSDS_Library Preparation Kit_Thermo Fisher
MSDS_Library Preparation Kit_MGI