Double Stranded cDNA Synthesis Kit
Stabilize RNA to double-stranded cDNA for unquestionable performance
Overview
Consistent cDNA Synthesis
for RNA Sequencing
The Celemics Double-stranded cDNA Synthesis Kit is designed to provide an optimized template for NGS-based RNA sequencing. The kit includes all necessary components for the process from first-strand synthesis to double-stranded cDNA enabling seamless NGS library preparation. With exceptional stability and performance, the kit enables accurate and efficient cDNA synthesis even from low-amount RNA samples, while significantly reducing reaction time.
cDNA Synthesis Workflow
- Sample amount : 25 ng to 1 μg (as low as 10 ng)*
- Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
- * Carrier RNA is required for sample amount < 25 ng
RNA Fragmentation
(Optional)
1st. Strand Synthesis
2nd. Strand Synthesis-1
2nd. Strand Synthesis-2
Double-stranded cDNA
of an RNA sample
cDNA Synthesis Workflow
- Sample amount : 25 ng to 1 μg (as low as 10 ng)*
- Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
- * Carrier RNA is required for sample amount < 25 ng
RNA Fragmentation
1st. Strand Synthesis
2nd. Strand Synthesis-1
2nd. Strand Synthesis-2
Double-stranded cDNA
of an RNA sample
cDNA Synthesis Workflow
- Sample amount : 25 ng to 1 μg (as low as 10 ng)*
- Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
- * Carrier RNA is required for sample amount < 25 ng
RNA Fragmentation (Optional)
1st. Strand Synthesis
2nd. Strand Synthesis-1
Double-stranded cDNA
of an RNA sample
2nd. Strand Synthesis-2
Double-stranded cDNA
of an RNA sample
Efficient cDNA Synthesis
from Low-amount RNA
Even with input as low as 10 ng, the kit consistently produces high-quality cDNA,
enabling accurate transcript representation and RNA-Seq results for limited or precious samples.
Reliable Synthesis from as Low as 10 ng of RNA
Library Size Distribution (Left) and Detected Viral Genome 1X Coverage (Right)
Demonstrated successful library construction and efficient genome detection from clinically derived viral samples with RNA inputs as low as 10 ng, confirming the kit’s robust performance even with limited material.
| Virus | SARS-CoV-2 | RSV A | RSV B | Influenza A | ||||
|---|---|---|---|---|---|---|---|---|
| Conc. (ng/μl) | 23.2 | 21.2 | 13.1 | 11.0 | 37.8 | 45.1 | 35.1 | 33.6 |
| Amount (ng) | 696 | 636 | 393 | 330 | 1134 | 1353 | 1053 | 1008 |
Reliable Performance
with High Reproducibility
Replicate experiments across different users demonstrated consistent yields and concentrations, confirming robustness and reproducibility. Compared to competitor kits, Celemics’ kit provides significantly higher cDNA yield under identical conditions.
Consistent Results Across Replicates and Users
Consistent cDNA yield and quality were observed across experiments performed by three different researchers, validating the kit’s reproducibility and robustness regardless of the operator.
| Input RNA | 10 ng | 100 ng | 500 ng | ||||||
|---|---|---|---|---|---|---|---|---|---|
| #1 | #2 | #3 | #1 | #2 | #3 | #1 | #2 | #3 | |
| Conc. (ng/μl) | 22.1 | 21.5 | 22.0 | 29.9 | 31.3 | 39.7 | 30.1 | 30.8 | 28.3 |
| Amount (ng) | 663 | 645 | 660 | 897 | 939 | 1191 | 903 | 924 | 849 |
Broad Compatibility
with Clinical and FFPE Samples
The kit demonstrated successful genome detection from clinical viral samples and fusion variant identification from FFPE RNA, proving its strong performance with difficult sample types.
Accurate Fusion Detection from FFPE RNA
Successfully detected all fusion variants from FFPE RNA using Seraseq® reference material, demonstrating the kit’s compatibility with degraded or challenging RNA inputs.
Library preparation results of triplicate samples
| Input Sample | 50 ng | ||
|---|---|---|---|
| Conc. (ng/μl) | 33.0 | 35.7 | 26.1 |
| Amount (ng) | 990 | 1071 | 783 |
| Target | Panel A | Panel B | Panel C | Target | Panel A | Panel B | Panel C |
|---|---|---|---|---|---|---|---|
| AKT1 | ○ | ○ | ○ | ALK | ○ | ○ | ○ |
| AR | ○ | ○ | ○ | BRAF | ○ | ○ | ○ |
| CDH1 | ○ | ○ | ○ | CDKN2A | ○ | ○ | ○ |
| CTNNB1 | ○ | ○ | ○ | EGFR | ○ | ○ | ○ |
| ERBB2 | ○ | ○ | ○ | ERBB3 | ○ | ○ | ○ |
| ESR1 | ○ | ○ | ○ | FGFR1 | ○ | ○ | ○ |
| FGFR2 | ○ | ○ | ○ | FGFR3 | ○ | ○ | ○ |
| HRAS | ○ | ○ | ○ | KRAS | ○ | ○ | ○ |
| MAP2K1 | ○ | ○ | ○ | MET | ○ | ○ | ○ |
| NOTCH1 | ○ | ○ | ○ | NRAS | ○ | ○ | ○ |
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Accurate identification of various ASFV strains in a single NGS reaction with species-specific blocker
Highly optimized, user- convenient NGS library preparation kit for all Celemics panels
Resources
Technical Resources
Safety Data Sheets
If you require the latest MSDS file, please contact us via ‘Contact Us‘.
MSDS_Double-Stranded cDNA Synthesis