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Double Stranded cDNA Synthesis Kit
Stabilize RNA to double-stranded cDNA for unquestionable performance
Overview
Double-Stranded cDNA Synthesis Kit
Celemics’ double stranded cDNA synthesis kit is designed to provide the optimized for template for NGS-based RNA sequencing.
The kit includes all components from RNA fragmentation to double stranded cDNA synthesis for NGS library preparation. The undeniable stability and performance of the kit allows for the cDNA synthesis even from low amounts of RNA samples with high accuracy and reduced reaction time.
cDNA Synthesis Workflow
- Sample amount : 25 ng to 1 μg (as low as 10 ng)*
- Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
- * Carrier RNA is required for sample amount < 25 ng
RNA Fragmentation
1st. Strand Synthesis
2nd. Strand Synthesis-1
2nd. Strand Synthesis-2
Double-stranded cDNA
of an RNA sample
cDNA Synthesis Workflow
- Sample amount : 25 ng to 1 μg (as low as 10 ng)*
- Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
- * Carrier RNA is required for sample amount < 25 ng
RNA Fragmentation
1st. Strand Synthesis
2nd. Strand Synthesis-1
2nd. Strand Synthesis-2
Double-stranded cDNA
of an RNA sample
cDNA Synthesis Workflow
- Sample amount : 25 ng to 1 μg (as low as 10 ng)*
- Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
- * Carrier RNA is required for sample amount < 25 ng
RNA Fragmentation
1st. Strand Synthesis
2nd. Strand Synthesis-1
Double-stranded cDNA
of an RNA sample
2nd. Strand Synthesis-2
Double-stranded cDNA
of an RNA sample
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Resources
Technical Resources
Safety Data Sheets
If you require the latest MSDS file, please contact us via ‘Contact Us‘.
MSDS_Double-Stranded cDNA Synthesis