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CeleNM™ Bead
Providing efficient method for normalizing your library
Overview
Enhance Your NGS Efficiency: Simple & Swift Protocol of CeleNM™ Bead
CeleNM™ Bead provides efficient method for normalization and assessment of the library.
It can replace QC process after the library preparation, which will allow a time and cost-saving NGS procedure throughout. Also, CeleNM™ Bead can provide robust and reproducible experimental results for quantity assessment compared to fluorescent dyes and electric signals by eliminating errors for detecting the actual fragments of interest.
Process for CeleNM™ Bead
Workflow
Library
Preparation
Purification
using
CeleNM Bead
Hybridization
Sequencing
and
Data Analysis
* CeleNM™ Bead to replace Library purification and QC.
Features & Benefits
Uniform Sequencing Data Distribution
CeleNM™ Bead replaces quantification and normalization process of prepared libraries in NGS procedure. In order to show the robustness and capability of normalization using CeleNM™ Bead, the sequencing data amounts were compared with the ones quantified and normalized using TapeStation and Qubit.
Distribution of sequenced raw base data for libraries quantified and normalized using Qubit, TapeStation and CeleNM™ Bead.
- Libraries assessed and normalized using CeleNM™ Bead demonstrates the lowest standard deviation with evenly distributed amount of sequencing data compared to Qubit and TapeStation (Agilent Technologies).
Sequencing data amount
Standard deviation
CeleNM™ Bead
TapeStation
Qubit
CeleNM™ Bead
TapeStation
Qubit
Method of Normalization
Sequencing data amount
CeleNM™ Bead
TapeStation
Qubit
Method of Normalization
Standard deviation
CeleNM™ Bead
TapeStation
Qubit
Method of Normalization
Comparable On-target Ratio and
Fold-80 Base Penalty
Multiplex samples normalized with CeleNM™ Bead shows comparable on-target ratio and fold-80 base penalty to single reaction, providing cost-effective solutions for producing quality NGS data.
Tested Superior Performance metrics:
on-target ratio and fold-80 base penalty.
- Comparison of on-target ratio and fold-80 base penalty of reaction versus samples normalized using CeleNM™ Bead demonstrated that CeleNM™ Bead can generate improved performance at lower cost.
On-target-ratio
Fold-80 base penalty
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