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High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides

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Nucleic Acid Research

High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides

Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, et al. High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides. Nucleic Acids Res. 2018 May 18;46(9):e55.

 

10.1093/nar/gky112