Methylation Sequencing Panel

Facilitate Epigenetics research with Celemics’ NGS solution

Overview

Methylation Sequencing

Methylation 시퀀싱은 유전체 전반적으로 또는 타겟 영역의 메틸화된 DNA의 위치와 수준을 식별하기 위한 방법으로 후성유전학 연구에서 주로 사용하는 시퀀싱 방법입니다. 셀레믹스의 Methylation 시퀀싱 솔루션은 유전체 전체 또는 특정 타겟 영역의 Methylation 패턴을 더 포괄적이고 상세히 제공할 수 있습니다.

Standard Workflow of Targeted Methyl-seq

Standard Workflow of Targeted Methyl-seq

Standard Workflow of Targeted Methyl-seq

DNA

Sample

Methylated 

adaptor

On-bead

conversion

protocol

Index PCR

Post-PCR

Library

Preparation

Bisulfite

Conversion

Target

Enrichment

Sequencing

Targeted

Methyl-seq Data

Standard Workflow of Targeted Methyl-seq

Celemics Proprietary Probe Design for Methyl-seq

Celemics Proprietary Probe Design for Methyl-seq

“G/A” panel

“C/T” panel

Pre-PCR

Bisulfite

conversion

Celemics Proprietary Probe Design for Methyl-seq

Celemics Proprietary Probe Design for Methyl-seq

Features & Benefits

Methyl-seq을 위해 특별히 고안된 프로브 사용

셀레믹스에서는 Bisulfite Conversion에 의한 시퀀스 변화를 고려하여 디자인한 프로브를 제공합니다. 이를 통해 Bisulfite Conversion 전후 시퀀스의 비교 분석을 수행하여 메틸화 부위를 정확하게 감지할 수 있습니다.

BI analysis for methylation sequencing

BI analysis for methylation sequencing

Alignment

FASTQ Filter

Methylation

Extractor

Visualization

Methylation

Pattern

Deduplication

Raw

Data

Bismark

Standard Analysis Pipeline Targeted Methyl-seq

BI analysis for methylation sequencing

다양한 샘플 타입, C/T Conversion 방법과 호환

셀레믹스의 메틸-시퀀싱 솔루션은 독자적인 프로브와 워크플로우를 사용하여 gDNA 및 cfDNA를 포함한 다양한 샘플에서 메틸화 분석을 수행할 수 있습니다.

효소 및 화학 처리를 통한 두 가지 C/T Conversion 방법 모두에 적용 가능하며 두 방법 사이에 시퀀싱 데이터에 차이가 나지 않는 최고 품질의 패널을 공급합니다.

High Reproducibilty of Methylation Pattern Analysis

The results demonstrate high reproducibility of the analysis, yielding the same methylation patterns when repeatedly tested with the identical specimens.

Related Products

related-product

NGS Library Preparation 키트로 셀레믹스의 Target Enrichment 패널에 최적화된 키트

Celemics Analysis Service

셀레믹스에서 제공하는 BI 분석 서비스로 다양한 수준의 분석 결과 제공 가능

Streptavidin Bead

최고 품질의 마그네틱 비드로 사용하기 편리하며 높은 재현성을 보여 NGS 실험에 최적화된 비드

Customized NGS Panel

고객의 목적에 맞게 유전자 영역을 자유롭게 구성한 NGS 패널로 시약부터 프로토콜까지 최적화된 패널 제공

Resources

Technical Resources

[Product Overview] Targeted Methylation Sequencing Panel

[Product Overview] Celemics Target Enrichment Panel Overview

[Catalogue] Celemics Products & Service Catalogue_All Products & Service

Safety Data Sheets

MSDS_Methylation Sequencing Panel_Illumina_Enzymeplus

MSDS_Methylation Sequencing Panel_Illumina

MSDS_Methylation Sequencing Panel_Thermo Fisher

MSDS_Methylation Sequencing Panel_MGI_Enzymeplus

MSDS_Methylation Sequencing Panel_MGI

References

Scientific Reports

Human epidermal growth factor receptor-2 expression and subsequent dynamic changes in patients with ovarian cancer

Kim Y-N, Chung YS, Park E, Lee ST, Lee J-Y. Human epidermal growth factor receptor-2 expression and subsequent dynamic changes in patients with ovarian cancer. Sci Rep. 2024;14(1).

 

10.1038/s41598-024-57515-y


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Translational Lung Cancer Research

Predicting disease recurrence in limited disease small cell lung cancer using cell-free DNA-based mutation and fragmentome analyses

Park S, Kang J-K, Lee N, Lee S-H, Kim H-P, Kim SY, et al. Predicting disease recurrence in limited disease small cell lung cancer using cell-free DNA-based mutation and fragmentome analyses. Transl Lung Cancer Res. 2024;13(2):280–91.

 

10.21037/tlcr-23-479


View Detail >

Turk J Gastroenterol

Clinical and Molecular Analysis in Patients with Peutz-Jeghers Syndrome

Aslan PG, Çağlayan AO, Bora E, Koç A, Yucel H, Ülgenalp A, et al. Clinical and molecular analysis in patients with peutz-jeghers syndrome. Gastrointestinal Endoscopy. 2024

 

10.5152/tjg.2024.23262


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Journal of Gynecologic Oncology

Application of precision medicine based on next-generation sequencing and immunohistochemistry in ovarian cancer: a real-world experience

Kim Y-N, Chung YS, Lee JH, Park E, Lee S-T, Kim S, et al. Application of precision medicine based on next-generation sequencing and immunohistochemistry in ovarian cancer: a real-world experience. J Gynecol Oncol. 2023;34(6).

 

10.3802/jgo.2023.34.e70


View Detail >