Double Stranded cDNA Synthesis Kit

Stabilize RNA to double-stranded cDNA for unquestionable performance

Overview

RNA 시퀀싱을 위한 안정적인 cDNA 합성

셀레믹스의 Double-stranded cDNA synthesis 키트는 NGS 기반 RNA 시퀀싱에 최적화된 Template를 제공하도록 제작되었습니다. 본 제품은 first-strand synthesis부터 이중가닥 cDNA(double-stranded cDNA) 생성까지 필요한 모든 시약을 포함하고 있어 NGS 라이브러리 제작 과정이 간편합니다. 뛰어난 안정성과 성능으로, 소량의 RNA 샘플에서도 정확하고 효율적인 cDNA 합성이 가능하여 반응 시간을 크게 단축할 수 있습니다.

cDNA Synthesis Workflow

Sample amount : 25 ng to 1 μg (as low as 10 ng)*
Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
* Carrier RNA is required for sample amount < 25 ng

RNA Fragmentation
(Optional)

1st. Strand Synthesis

2nd. Strand Synthesis-1

2nd. Strand Synthesis-2

Double-stranded cDNA

of an RNA sample

cDNA Synthesis Workflow

Sample amount : 25 ng to 1 μg (as low as 10 ng)*
Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
* Carrier RNA is required for sample amount < 25 ng

RNA Fragmentation

1st. Strand Synthesis

2nd. Strand Synthesis-1

2nd. Strand Synthesis-2

Double-stranded cDNA

of an RNA sample

cDNA Synthesis Workflow

Sample amount : 25 ng to 1 μg (as low as 10 ng)*
Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
* Carrier RNA is required for sample amount < 25 ng

RNA Fragmentation

1st. Strand Synthesis

2nd. Strand Synthesis-1

Double-stranded cDNA
of an RNA sample

2nd. Strand Synthesis-2

Double-stranded cDNA
of an RNA sample

Features & Benefits

소량의 RNA로도 효율적인 cDNA 합성

단 10 ng의 RNA로 고품질의 cDNA를 안정적으로 합성할 수 있어, 귀중하거나 제한된 샘플에서도 정확한 전사체 분석 및 RNA-Seq 결과를 제공합니다.

10 ng RNA에서도 안정적인 cDNA 합성 가능

Library Size Distribution (Left) and Detected Viral Genome 1X Coverage (Right)

Demonstrated successful library construction and efficient genome detection from clinically derived viral samples with RNA inputs as low as 10 ng, confirming the kit’s robust performance even with limited material.

Virus	SARS-CoV-2				RSV A	RSV B	Influenza A
Conc. (ng/μl)	23.2	21.2	13.1	11.0	37.8	45.1	35.1	33.6
Amount (ng)	696	636	393	330	1134	1353	1053	1008

높은 재현성과 신뢰할 수 있는 성능

사용자 간 반복 실험에서도 수율과 농도 면에서 일관된 결과를 보여, 키트의 우수한 안정성과 재현성을 입증했습니다. 동일한 조건에서 타사 제품과 비교했을 때, 셀레믹스의 키트는 높은 cDNA 수율을 제공합니다.

사용자와 반복 실험 간에도 일관된 결과

Consistent cDNA yield and quality were observed across experiments performed by three different researchers, validating the kit’s reproducibility and robustness regardless of the operator.

Input RNA	10 ng			100 ng			500 ng
Input RNA	#1	#2	#3	#1	#2	#3	#1	#2	#3
Conc. (ng/μl)	22.1	21.5	22.0	29.9	31.3	39.7	30.1	30.8	28.3
Amount (ng)	663	645	660	897	939	1191	903	924	849

임상 샘플 및 FFPE 샘플과의 뛰어난 호환성

임상 유래 바이러스 샘플에서의 유전체 검출과 FFPE RNA에서의 fusion variant identification에 성공하여 분석이 어려운 샘플에서도 높은 성능을 입증했습니다.

FFPE RNA에서도 정확한 Fusion Detection 가능

Successfully detected all fusion variants from FFPE RNA using Seraseq® reference material, demonstrating the kit’s compatibility with degraded or challenging RNA inputs.

Library preparation results of triplicate samples

Input Sample	50 ng
Conc. (ng/μl)	33.0	35.7	26.1
Amount (ng)	990	1071	783

Fusion Detection Results

Target	Panel A	Panel B	Panel C	Target	Panel A	Panel B	Panel C
AKT1	○	○	○	ALK	○	○	○
AR	○	○	○	BRAF	○	○	○
CDH1	○	○	○	CDKN2A	○	○	○
CTNNB1	○	○	○	EGFR	○	○	○
ERBB2	○	○	○	ERBB3	○	○	○
ESR1	○	○	○	FGFR1	○	○	○
FGFR2	○	○	○	FGFR3	○	○	○
HRAS	○	○	○	KRAS	○	○	○
MAP2K1	○	○	○	MET	○	○	○
NOTCH1	○	○	○	NRAS	○	○	○

Resources

Technical Resources

[Product Sheet] Celemics Double-stranded cDNA Synthesis Kit

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Celemics Target Enrichment Panel Overview

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Celemics Products & Services

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Safety Data Sheets

최신 MSDS 파일이 필요하시면 ‘Contact Us‘를 통해 문의 주시기 바랍니다.

MSDS_Double-Stranded cDNA Synthesis

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