CeleNM Bead

Providing efficient method for normalizing your library

Overview

빠르고 간편한 프로토콜로
NGS 효율을 높인 CeleNM Bead

CeleNM Bead는 라이브러리 준비 후 QC 프로세스를 대체할 수 있어 NGS 실험에 필요한 시간과 비용을 절약할 수 있습니다또한, CeleNM Bead는 실제 관심 대상 조각(the actual fragments of interest)을 감지하는데 발생하는 오류를 제거함으로써 Fluorescent dyes 및 Electric signals보다 견고하고 재현성있는 Quantity Assessment의 결과를 확인할 수 있습니다.

Process for CeleNM Bead
Workflow

Library
Preparation

Purification
using
CeleNM Bead

Hybridization

Sequencing
and
Data Analysis

* CeleNM Bead to replace Library purification and QC.

Features & Benefits

균일한 시퀀싱 데이터 분포

CeleNM Bead는 NGS 프로세스에서 라이브러리의 정량(Quantification)과 정규화(Normalization) 과정을 대체합니다. CeleNM Bead를 사용한 정량 및 정규화된 라이브러리는 TapeStation Qubit에 비해 시퀀싱 데이터 양이 균등하게 분산되고, 가장 낮은 표준 편차를 나타내는 것을 통해 CeleNM Bead 우수한 성능을 확인할 수 있습니다.

Distribution of sequenced raw base data for libraries quantified and normalized using Qubit, TapeStation and CeleNM Bead.

  • Libraries assessed and normalized using CeleNM Bead demonstrates the lowest standard deviation with evenly distributed amount of sequencing data compared to Qubit and TapeStation (Agilent Technologies).

Sequencing data amount

Standard deviation

CeleNM Bead

TapeStation

Qubit

CeleNM Bead

TapeStation

Qubit

Method of Normalization

Sequencing data amount

CeleNM Bead

TapeStation

Qubit

Method of Normalization

Standard deviation

CeleNM Bead

TapeStation

Qubit

Method of Normalization

우수한 On-target Ratio 및
Fold-80 Base Penalty

CeleNM Bead로 정규화된 멀티플렉스(Multiplex) 샘플은 단일(Singleplex) 반응의 온타겟(On-target) 비율과 Fold-80 Base Penalty에서 유사한 성능을 나타냄으로써 품질 높은 NGS 데이터를 생산하는 비용 효율적인 솔루션을 제공합니다.

Tested Superior Performance metrics:
on-target ratio and fold-80 base penalty.

  • Comparison of on-target ratio and fold-80 base penalty of reaction versus samples normalized using CeleNM™ Bead demonstrated that CeleNM™ Bead can generate improved performance at lower cost.

On-target-ratio

Fold-80 base penalty

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Resources

Technical Resources

[Product Sheet] CeleNMBead

[Product Overview] Celemics Accessories

Celemics Target Enrichment Panel Overview

Celemics Products & Services