Overview
Immune Repertoire often represents an individual’s current immuno-
logical status, whether healthy, vaccinated, diseased or infected.
immune repertoire. Celemics presents Immune Repertoire Profiling Service, providing effective data
acquisition, integration, and interpretation for the customers.
Features &
Benefits
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- Quantitative analysis of the library diversity
- NGS-based analysis of complex antibody library consisting of millions
(106 ~ 1012) of sequences in a single experiment - Analysis of immunoglobulin and T-cell receptor repertoire
analysis of BCR/TCR for each clone - Frequency analysis of individual antibody clones within the library,
identifying major and minor clones
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- Tracking of clonal frequencies for each sample
- For antibody discovery, analysis of library diversity according to
its panning degree enabling monitoring changes in clonal frequency - Minimized omission of potentially significant antibody clones
- Analysis of immune repertoire characteristics from blood
sample and monitoring of each clone
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- Various analysis options for immune system studies
- Perform the experiment with drastically reduced time and cost enabled
by the advanced technology of MSSIC developed from Celemics
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- Support various types of BI data analysis
- Celemics’ proprietary BI analysis pipeline provides the various, visible,
and easy to read results for customer’s antibody libraries.
Workflow
General workflow of Immune Repertoire Profiling service
Celemics provides service for data acquisition, integration and analysis, and interpretation.
Example of analysis report
Discovery of candidate antibodies from actively immunized chickens
| CDR3 ID | CDR3 AA | p_pre- immune |
p_immune | p_1st_boost | p_2nd_boost | p_tissue | p_BM_2nd |
|---|---|---|---|---|---|---|---|
| CDR3sample_1 | GSRDSSASTI | 2433 | 773 | 31 | 0 | 2 | 461 |
| CDR3sample_2 | GSYDSSYVGI | 1756 | 1444 | 2269 | 895 | 1058 | 789 |
| CDR3sample_3 | GSIDSSYVGI | 1019 | 402 | 876 | 938 | 541 | 346 |
| CDR3sample_4 | ANFDSSSGAGI | 46 | 25 | 338 | 483 | 1707 | 345 |
| CDR3sample_5 | GGYDSSAGI | 231 | 268 | 934 | 207 | 966 | 7770 |
| CDR3sample_6 | GSFDSSTYAGI | 3678 | 1034 | 425 | 290 | 547 | 431 |
| CDR3sample_7 | GSRDSSASTI | 2433 | 773 | 31 | 0 | 2 | 461 |
| CDR3sample_8 | GSRDSSYVGI | 6427 | 6370 | 10151 | 5756 | 10089 | 2680 |
| CDR3sample_9 | GGYDGSTYVGI | 279 | 211 | 2047 | 178 | 271 | 88 |
| CDR3sample_10 | GSRDSNYVGI | 407 | 567 | 974 | 749 | 868 | 224 |
| CDR3sample_11 | GSSSGTGI | 1563 | 2580 | 899 | 1999 | 114 | 24702 |
| CDR3sample_12 | GSYDSSAGI | 1195 | 875 | 1342 | 743 | 746 | 288 |
| CDR3sample_13 | GSRDSTYVGI | 461 | 795 | 1355 | 998 | 983 | 136 |
| CDR3sample_14 | GGYDSSTDAGI | 1167 | 1129 | 1353 | 1617 | 892 | 405 |
Analysis Report
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- Antibody Clustering by sequence
- With the clustering analysis, customers can obtain the
immunizing status of the sample.
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- Example of network analysis
- Customers can easily recognize the relationship among the antibody
library with visible network analysis
Example of network analysis
Provide information of physical antibody status to select the target of the drug candidate
Applications
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Discovery of novel drug candidate
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Researches for Immunization