Double-Stranded cDNA Synthesis Kit is optimized for NGS-based RNA sequencing.The kit includes all components from RNA fragmentation to double-stranded cDNA synthesis for NGS library preparation.
The robust performance of the kit allows for the cDNA synthesis even from low amounts of RNA samples with high accuracy and reduced reaction time.
cDNA SYNTHESIS WORKFLOW
Sample amount : 25 ng to 1 μg (as low as 10 ng)*
Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
* Carrier RNA is required for sample amount < 25 ng