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Comprehensive Respiratory Virus Panel

Superior Success Rate with Low Amounts of Clinical Sample

COVID-19 WGS with Celemics Comprehensive Respiratory Virus Panel

Overview

SARS-CoV-2 Phylogenetic Tree

※ Variants of global importance highlighted in bold.

Detection panel for the accurate sequencing and identification of prominent whole viral genomes, including SARS-CoV-2 mutations and variants (ex. Omicron, Delta).

Viruses are continuously evolving in ways that make them increasingly infectious and difficult to cure. Thus, the discovery of specific disease-causing viral strains and research into their ability to spread within individuals in a given population have become a paramount public health issue.

The Celemics Comprehensive Respiratory Virus Panel (CRV Panel) was developed to detect and sequence respiratory disease-causing viruses in humans using the NCBI RefSeq database as its foundation.

It allows for the Whole Genome Sequencing of SARS-CoV-2 and all its relevant mutations and variants, and enables simultaneous testing of 9 different virus types and its 39 strains of clinically significant and prevalent respiratory viruses.

※ Global Distribution of SARS-CoV-2 Variants.

Performance

“Excellent and high coverage from clinical samples using CRV Panel”

The performance test results from clinical samples show excellent Whole Genome coverage.

Performance
Sample Type Coverage (1X) Coverage (10X) Coverage (100X)
Clinical specimen
( Illumina 2 X 75bp )
99.95% 99.87% 98.95%

※ Mutation Frequency and Genomic Structure of SARS-CoV-2 Variants.

Features &
Benefits

SARS-CoV-2 WGS Provision
Provision of whole genome sequences for
SARS-CoV-2 and all related mutations
Variant detection also possible
Superior WGS success rate even with poor
quality specimen
Able to detect pathogens from patient specimens as well as
environment specimens even with poor quality
Exceptional success rate of variant detection and WGS
Significantly reduced gap formation
Coverage of wide range of respiratory pathogens
Assess WGS of 39 strains for 9 different virus types
(SARS-CoV-2 solo analysis is also possible)
Includes all types of respiratory virus that are assessed
by medical institutions around the globe
Double pandemic / coinfection detection
possible in single assay
Inclusion of stand-alone SW for BI analysis
Receive stand-alone SW for BI analysis

Specification

“CRV Panel Specification”

Specification
Target Virus 9 types / 39 virus strains, including SARS-CoV-2
Mutation Analysis Variant detection, Viral mutation (SNV, Indel) from generated Whole Genome Sequence
Sample Type URT, NP/OP, etc.
Platform All Illumina sequencers
Kit Composition Provides all required reagents, including RNA to cDNA kit, cDNA to captured library kit, and bioinformatics SW
Bioinformatics Pipeline Stand-alone bioinformatics SW ‘Celemics Virus Verifier’ ( FASTQ to Visual Report )

Pathogen list

“CRV Panel Pathogen list”

Pathogen list
Virus Strain
Coronavirus Coronavirus HKU1
Coronavirus NL63
Coronavirus 229E
Coronavirus OC43
SARS-CoV-2
Bocavirus 1/2/3/4/ (HBoV) Human Bocavirus 1
Human Bocavirus 2
Human Bocavirus 3
Human Bocavirus 4
Human Rhinovirus (A/B/C) Human Rhinovirus A
Human Rhinovirus B
Human Rhinovirus C
Human Adenovirus Human Adenovirus Type 1 (HAdV-C1)
Human Adenovirus Type 2 (HAdV-C2)
Human Adenovirus Type 3 (HAdV-B3)
Human Adenovirus Type 4 (HAdV-E4)
Human Adenovirus Type 5 (HAdV-C5)
Human Adenovirus 7 (HAdV-B7)
Human Adenovirus 14 (HAdV-B14)
Human Adenovirus 21 (HAdV-B21)
Pathogen list
Virus Strain
Human Enterovirus EV-C104
EV-C105
EV-C109
EV-C117
EV-C118
CV-A21
EV-D68
Influenza A Influenza A Virus (Flu A)
Influenza A-H1 Virus (Flu A-H1)
Influenza A-H3 Virus (Flu A-H3)
Influenza B Influenza B Virus (Flu B)
Parainfluenza Virus Parainfluenza 1 (PIV 1)
Parainfluenza 2 (PIV 2)
Parainfluenza 3 (PIV 3)
Parainfluenza 4 (PIV 4) A
Parainfluenza 4 (PIV 4) B
Respiratory Syncytial Virus Respiratory Syncytial Virus A (RSV A)
Respiratory Syncytial Virus B (RSV B)
Human Metapneumovirus
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Stand-alone
SW

“Accurate Results from BI analysis software developed by Celemics”

※ Celemics provides stand-alone SW for BI analysis, allowing customers to access detailed data analysis information and ensuring the security of client sequence information.

Workflow

01

RNA extraction

02

NGS Iibrary Preparation

cDNA synthesis, NGS Prep.

~5 hours

03

Target enrichment

Hybridization capture

~ 4 hours

04

Sequencing

Illumina sequencer

Depending on read length

05

Data analysis

Virus Data Report

~ 3 hours

Inclusive preparation kit provided directly from Celemics

  • Portions of the procedure covered through the Complete Kit are highlighted in Red
  • Competitors: 16 hours (Target Enrichment step, Hybridization Capture)